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smooth muscle nucleofection solution  (Lonza)


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    Lonza smooth muscle nucleofection solution
    Smooth Muscle Nucleofection Solution, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smooth+muscle+nucleofection+solution/basic+nucleofector+kit+primary+mammalian+smooth+muscle+cells/pmc05086536-54-11-14
    Average 90 stars, based on 1 article reviews
    smooth muscle nucleofection solution - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Human Parturition Involves Phosphorylation of Progesterone Receptor-A at Serine-345 in Myometrial Cells
    Article Snippet: Thus, the pSer345 antibody detects pSer344- and pSer345-PR, and as such it is possible that phosphorylation at both sites exists in the pregnancy myometrium. hTERT-HM and hTERT-HM A/B cells were transiently transfected with expression plasmids or siRNAs by nucleofection (Lonza Basal, Switzerland). .. Briefly, cells were harvested by trypsinization, centrifuged, and resuspended in smooth muscle nucleofection solution (Lonza; catalog number VPI-1004) to a concentration of 1 × 10 6 cells per 100 μL containing DNA or siRNA to be transfected. .. The mixture was then transferred to an electroporation cuvette, placed in the Nucleofector device (Lonza), and subjected to program A33.

    Transfection:

    Article Title: Human Parturition Involves Phosphorylation of Progesterone Receptor-A at Serine-345 in Myometrial Cells
    Article Snippet: Thus, the pSer345 antibody detects pSer344- and pSer345-PR, and as such it is possible that phosphorylation at both sites exists in the pregnancy myometrium. hTERT-HM and hTERT-HM A/B cells were transiently transfected with expression plasmids or siRNAs by nucleofection (Lonza Basal, Switzerland). .. Briefly, cells were harvested by trypsinization, centrifuged, and resuspended in smooth muscle nucleofection solution (Lonza; catalog number VPI-1004) to a concentration of 1 × 10 6 cells per 100 μL containing DNA or siRNA to be transfected. .. The mixture was then transferred to an electroporation cuvette, placed in the Nucleofector device (Lonza), and subjected to program A33.



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    <t>Nucleofection</t> with siNOX2 significantly reduced NOX2 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (A, n = 5–9). Similarly, nucleofection with siNOX4 significantly reduced NOX4 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (B, n = 5–9). *P<0.05 vs control.
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    <t>Nucleofection</t> with siNOX2 significantly reduced NOX2 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (A, n = 5–9). Similarly, nucleofection with siNOX4 significantly reduced NOX4 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (B, n = 5–9). *P<0.05 vs control.
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    <t>Nucleofection</t> with siNOX2 significantly reduced NOX2 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (A, n = 5–9). Similarly, nucleofection with siNOX4 significantly reduced NOX4 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (B, n = 5–9). *P<0.05 vs control.
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    Nucleofection with siNOX2 significantly reduced NOX2 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (A, n = 5–9). Similarly, nucleofection with siNOX4 significantly reduced NOX4 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (B, n = 5–9). *P<0.05 vs control.

    Journal: PLoS ONE

    Article Title: Upregulation of Intermediate-Conductance Ca 2+ -Activated K + Channels (KCNN4) in Porcine Coronary Smooth Muscle Requires NADPH Oxidase 5 (NOX5)

    doi: 10.1371/journal.pone.0105337

    Figure Lengend Snippet: Nucleofection with siNOX2 significantly reduced NOX2 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (A, n = 5–9). Similarly, nucleofection with siNOX4 significantly reduced NOX4 expression but did not prevent upregulation of KCNN4 mRNA expression by bFGF (25 ng/mL) in CSMCs (B, n = 5–9). *P<0.05 vs control.

    Article Snippet: 4 day serum-starved cultured CSMCs were trypsinized, placed in basic smooth muscle nucleofection solution (Amaxa), and transfected with pAP-1 Luc Cis-Reporter plasmid in which firefly luciferase gene expression in the reporter plasmid is controlled by a synthetic promoter containing direct repeats of the AP-1 transcription recognition sequence (1.0ug, Stratagene 219074).

    Techniques: Expressing, Control